Freshly Isolated Human Cells

We are continually adding to our range of cells isolated from fresh human tissue. These are a valuable, physiologically relevant in vitro research tool. We can offer:

  • Primary Cells and cell lines
  • Normal/healthy human cells
  • Disease specific human cells

These can be shipped as live cultures or cryopreserved.

Hepatocytes:

  • Fresh or Frozen,
  • For growing in suspension or in monolayers,
  • As short— (5 to 6 days) or long—term cultures (up to 3 weeks).
  • Fresh cells can be shipped in flasks or multiwall plates.

All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

Peripheral Blood Mononuclear Cells (PBMC):

Cryopreserved human PBMCs and Cord Blood Mononuclear Cells are now available immediately, with functionality comparable to fresh isolations. We also offer highly purified monocyte, T-cells and B-cell subsets. These are available with basic or enhanced Characterization.

  • PBMCs available from normal and diseased donors (includes autoimmune diseases: RA, SLE,
    Crohn’s, MS)
  • Basic Characterization — includes age, gender, ethnicity, HLA-I data
  • Enhanced Characterization — includes age, gender, ethnicity, HLA Class-I and Class-II data and
    ELISpot-IFNγ responses to PHA, CEF and CMV peptide pools

All cells are supplied with donor information including age, sex, liver pathology and medical history. We can also supply subcellular fractions: microsomes, S9 fractions (non—induced or induced and cytosols).

Human Keratinocytes:

Normal adult keratinocytes prepared from breast or abdominal skin.

Human Fibroblasts:

Isolated from abdominal skin, breast skin or scalp samples.

Human Melanocytes:

Epidermal melanocytes isolated from the skin of healthy donors.

Human Adipocytes & Pre—adipocytes:

Subcutaneous adipocytes and pre—adipocytes purified from surgical excess normal material. Protocols for culture and differentiation are provided.

Human Pancreatic Islets:

High quality, characterised human pancreatic islets are available for research use, including normal human islets and pancreatic islets from type 1 and type 2 diabetic donors.

All our human cells have been isolated from donors that are negative for HIV1/2, HCV and HBV infection and certified free of mycoplasmal, bacterial, yeast and fungal contamination. Please contact us for details of our current stock availability.

 

For more information please contact us:

Email: enquiries@tissue-solutions.com
Tel: +44 (0)141 299 0055 Fax: +44 (0)141 299 0054